DNA

Part:BBa_K582003:Design

Designed by: Mitesh Agrawal   Group: iGEM11_GeorgiaTech   (2011-09-28)


Cas 9 + Repeat/Spacer Region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 4698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4698
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 4698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4698
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3202
    Illegal BsaI.rc site found at 4173


Design Notes

The sequence has the restriction sites present on the pSB1C3 backbone. We will have to subject it to mutagenesis to make the biobrick functional.


Source

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 for Cas9

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=genbank&from=648900&to=650325 for Repeat/Spacer Region

References